(STEM) CELL FATE AND FUNCTION
currently studying functional roles for post-transcriptional regulation in shaping cell fate in early development
Post-transcriptional regulation of Profilin-2 by microRNAs and RNA-binding proteins forms a critical regulatory node for early embryonic cell fate decisions. (2026)
Here, we show that coordinate RBP and microRNA control of a single transcript, Profilin 2 (Pfn2), is essential for differentiation of embryonic stem cells (ESCs) into the primary germ layer lineages.
We discover that the choreographed microRNA-IRE axis of control on the Pfn2 transcript is essential for two key signal transduction steps during ESC differentiation.
POST-TRANSCRIPTIONAL REGULATION IN PLURIPOTENT STEM CELL BIOLOGY AND PLURIPOTENT STATE TRANSITION
defined an axis of post-transcriptional control, endocytosis, and signal transduction that is essential for stem cell growth, cell cycle control, and early differentiation
embryoid bodies undergoing cavitation
METHODS PUBLICATIONS (STEM-CELL FATE AND FUNCTION RELATED)
Measuring Endocytosis and Endosomal Uptake at Single Cell Resolution. (2022)
Endocytosis impacts many cell biological functions, including in embryonic stem cells (ESCs). It has been shown that endocytosis is necessary for adequate FGF-signaling within the preimplantation ESC to post-implantation epiblast (EpiLC) pluripotency continuum and is required for proper levels of ERK activation. Quantitative methods at single cell resolution are needed to study endocytosis as well as its regulation and roles in these transitioning populations. The methods in this chapter provide an easily adaptable, multiplexable platform to monitor and quantify endosomal uptake at single cell resolution in live cells following receptor-mediated and non-receptor-mediated endocytosis, including nonspecific mechanisms such as pinocytosis.
